A Biological Safety Cabinet (BSC), also known as a Biosafety Cabinet is mainly used for
handling pathogenic biological samples or for applications that require a sterile work zone. A
biological safety cabinet creates inflow and downflow of air that provides operator protection.
The downflow air passes through an ULPA/HEPA filter and creates an ISO Class 3 work zone
to protect samples from the risk of cross-contamination. The air exhausted also passes
through an ULPA/HEPA filter prior to release to protect the environment.
Whitenair biosafety cabinets have a wide range of options from work trays, side panel
construction to accessories. Whitenair BSCs are designed with ergonomic and energy-saving
features.
To guarantee the biological safety cabinet's optimum performance these are tested and
classified by 2 standards: EN 12469:2000 and NSF 49.
Class I Biosafety Cabinet
A Class I Biosafety cabinet(BSC) protects the operator and the environment from
exposure to biohazards. It does not prevent samples being handled in the cabinet
from being exposed to contaminants that may be present in room air. Naturally,
there is a possibility of cross-contamination that may affect experimental
consistency. Consequently, the scope and application of Class I cabinets is limited,
and it is largely considered obsolete.
Model No
External Working Area
Internal Working Area
L
D
H
L1
D1
H1
W BSC 30 C1
1340
735
2150
1220
660
670
Class II Biosafety Cabinets are Two Types Class II A2 and Class II B2
Class II A2
Whitenair Class II Type A2 biological safety cabinet is the most common Class II cabinet. It has a
plenum from which 30% of air is exhausted, and 70% re-circulated to the work area as the
downflow.
Stated from NSF/ ANSI 49:2010, both the Class II Type A2 must have the positively-pressurized
contaminated plenum to be surrounded by negative pressure. In case there is a leakage on the
positive plenum, the leaking aerosol will be pulled by the negative pressure back to the positive
plenum, and it will not leak out.
In the A2 cabinet, about 70% of air from the positive plenum is recirculated as downflow, and the
remaining 30% is discharged to the lab through the exhaust filter.
Model No
External Working Area
Internal Working Area
L
D
H
L1
D1
H1
W BSC 30 A2
1050
905
2251
915
610
610
W BSC 40 A2
1350
905
2251
1220
610
610
W BSC 50 A2
1800
905
2251
1525
610
610
W BSC 60 A2
2400
905
2251
1830
610
610
Model No
External Working Area
Internal Working Area
L
D
H
L1
D1
H1
W BSC 30 B2
1250
905
2251
915
610
610
W BSC 40 SB2
1500
905
2251
1220
610
610
W BSC 50 B2
1850
905
2251
1525
610
610
W BSC 60 B2
2100
905
2251
1830
610
610
Class II B2
Whitenair Class II Type B2 biosafety cabinets are suitable for work with toxic chemicals employed as
an adjunct to microbiological processes under all circumstances since no re-circulation occurs. In
theory, Type B2 biological safety cabinets may be considered as the safest of all Class II BSCs since the
total exhaust feature acts as a fail-safe in the event that the downflow and / or exhaust HEPA
filtration systems cease to function normally. However, Class II Type B2 biosafety cabinets require
large laboratory spaces due to their installation system and will require elaborate ducting works.
Class III
Whitenair Class III biological safety cabinets are suitable for work with microbiological agents
assigned to biosafety levels 1, 2, 3 and 4. They are frequently specified for work involving the most
lethal biological hazards.
Work is performed through glove ports in the front of the cabinet. During routine operation, negative
pressure relative to the ambient environment is maintained within the biosafety cabinet. This provides
an additional fail-safe mechanism in case physical containment is compromised. Exhaust air is usually
HEPA filtered and incinerated. Alternatively, double HEPA filtration with two filters in series may be
utilized.
Model No
External Working Area
Internal Working Area
L
D
H
L1
D1
H1
W BSC 40 C3
1690
850
2270
1130
560
635
W BSC 50 C3
1500
850
2270
1430
560
635
W BSC 60 C3
1800
850
2270
1755
560
635
Whitenair Technologies, being established in the year 2000, is engaged in serving its clients with an impeccable range.